Single-Molecule Assay of Biological Reaction in Femtoliter Chamber Array
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概要
- 論文の詳細を見る
We previously developed micron-sized, femtoliter (fL) reaction chamber array made of polydimethylsiloxane and polyacrylamide. Downsizing of the reaction volume to fL level made it possible to carry out single-molecule assay (SMA) of biological reactions in a straightforward manner. In this review, preparation of the fL chamber array and its application to SMA of enzyme, DNA, and rotary motor protein under an optical microscope are described.
- 2009-08-25
著者
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Iino Ryota
The Institute Of Scientific And Industrial Research Osaka University
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Tabata Kazuhito
The Institute of Scientific and Industrial Research, Osaka University
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Noji Hiroyuki
The Institute Of Industrial And Scientific Research Osaka University
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Lam Liza
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
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Tabata Kazuhito
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
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Rondelez Yannick
Institute of Industrial Science, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153-8505, Japan
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Iino Ryota
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
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Noji Hiroyuki
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan
関連論文
- 2P186 Single-molecule measurement of F_0F_1 rotation in living E. coli(The 48th Annual Meeting of the Biophysical Society of Japan)
- 1P180 Development of a force-clamping system to study the torque generation mechanism of F_1-ATPase(Molecular motor,The 48th Annual Meeting of the Biophysical Society of Japan)
- 3P-129 1分子回転観察におけるV_1-ATPaseの不活性化の解析(分子モーター(3),第46回日本生物物理学会年会)
- 2P-123 シンプルな光学系で高時間・高空間分解能を実現する暗視野顕微鏡の開発(分子モーター,第47回日本生物物理学会年会)
- 2P178 Mechanical Reactivation from Long Pause State of V_1-ATPase(The 48th Annual Meeting of the Biophysical Society of Japan)
- 1P-259 バクテリア-細胞レベルでの細胞内ATPイメージング(バイオイメージング,第47回日本生物物理学会年会)
- 1TP4-10 Angle Dependency of ATP binding event in V1-ATPase(The 47th Annual Meeting of the Biophysical Society of Japan)
- 3P-147 F1-ATPaseによる基質加水分解機構の解明(分子モーター(3),第46回日本生物物理学会年会)
- 2P-191 重水中でのF_1-ATPaseの1分子回転観察(分子モーター(2),第46回日本生物物理学会年会)
- 1P301 ε Subunit Stops Rotation of Thermophilic F1-ATPase; Single Molecular Analysis of the inhibition by the ε subunit(9. Molecular motor (I),Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- 2P-128 F_1-ATPaseのβサブユニットのC末ドメインとγサブユニットの相互作用に関する研究(分子モーター,第47回日本生物物理学会年会)
- Single-Molecule Assay of Biological Reaction in Femtoliter Chamber Array