Separation-Type Multiplex Polymerase Chain Reaction Chip for Detecting Male Infertility

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概要

• 論文の詳細を見る

• A novel polymerase chain reaction (PCR) biochip is presented in this paper. In this PCR chip, the glass substrate integrated with the microheater and microsensor is separable from the reaction chamber where the sample is injected, which now makes repeated reuse of the glass substrate possible. The heat transfer efficiency and target gene amplification of the proposed separable PCR chip was compared with that of the conventional united PCR chip. The results showed that the sex-determining Y chromosome (SRY) gene PCR for detecting male infertility was successfully performed in the separable chip. However, repeated multiplex PCR was successful for only two genes, SPGY1 and SRY, but not for gene SY586. Future work is needed for a multiplex PCR with more than three genes.

• 2008-06-25

著者

• Hwang Seung

  Division Of Applied Life Science Graduate School Gyeongsang National University:research Institute O

• Ahn Yoomin

  Department Of Mechanical Engineering Hanyang University

• Ju Jin-Kyoung

  Department of Mechanical Engineering, Graduate School, Hanyang University, Seoul 133-791, Korea

• Ha Seung-Mo

  Department of Mechanical Engineering, Graduate School, Hanyang University, Seoul 133-791, Korea

• Ahn Yoomin

  Department of Mechanical Engineering, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea

• Hwang Seung

  Division of Molecular and Life Science, Hanyang University, Ansan, Gyeonggi-do 426-791, Korea

• HA Seung-Mo

  Department of Mechanical Engineering, Graduate School, Hanyang University

関連論文

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• Separation-Type Multiplex Polymerase Chain Reaction Chip for Detecting Male Infertility
• Lateral Crack in Abrasive Wear of Brittle Materials (Solid Mechanics and Material Engineering)
• Separation of Progressive Motile Sperm from Mouse Semen Using On-chip Chemotaxis

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