Effects of Iron-cored Carbonballs as Matrix on Matrix-Assisted Laser Desorption Ionization-time of Flight Mass Spectrometer Analysis
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概要
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Mass spectrometry is an important technique for characterizing the structures of surfaces and has several characteristics that are especially valuable in the bioanalytical application. Matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS) is commonly used in preliminary protein identification. To prepare the sample for analysis, purified-proteins of cryptic digestion are carefully mixed with saturated liquid-phase matrices, classified as high-molecular-weight organic polymer, which are essential to promote analytical accuracy via mechanism of laser-energy transfer to peptides. Signals generated from matrix in the analytical process interfere with signals of small peptides in the same region of mass-to-charge ratio. In order to improve analytical results, we demonstrated that matrix in MALDI-TOF-MS can be replaced by carbon nanomaterial. We substituted iron-cored carbonballs for matrix well mixing with trypsinized bovine serum albumins (BSA) and performed mass spectrometrical analyses. Experimental results indicated that in addition to iron-cored carbonballs possessed equivalent functionality as matrix does, several improved aspects were also observed.
- 2007-09-30
著者
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Tsai Laifu
National Nano Device Laboratories, Southern Region Office, Tainan, Taiwan, R.O.C.
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Liao Paochi
Proteomics Core Research Laboratory, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
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Tyan Yuchang
Proteomics Core Research Laboratory, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.
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Wen Hsiangshen
National Nano Device Laboratories, Southern Region Office, Tainan, Taiwan, R.O.C.
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Liu Chienwei
National Nano Device Laboratories, Southern Region Office, Tainan, Taiwan, R.O.C.
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Shy Shyilong
National Nano Device Laboratories, Southern Region Office, Tainan, Taiwan, R.O.C.
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Dai Bautong
National Nano Device Laboratories, Southern Region Office, Tainan, Taiwan, R.O.C.
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Wang Chongyao
Proteomics Core Research Laboratory, College of Medicine, National Cheng Kung University, Tainan, Taiwan, R.O.C.