電気インピーダンストモグラフィ（EIT）を用いた細胞凍結過程の可視化に関する基礎的検討

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概要

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• We report the results of a preliminary trial to visualize a cell during freezing process using microscopic electrical impedance tomography (micro-EIT). A printed circuit board was developed for micro-EIT with two measurement chambers, 5mm in diameter and 1.6mm in depth. These chambers were filled with ethanol (2mS/cm) which was then kept at -30°C by placing it on a copper block filled with liquid nitrogen and by using a heater with a programmable controller. A grain of Citrus Sudachi and an egg of Cypselurus Agoo Agoo were placed into the measurement chamber and were recorded data during freezing by using a CCD microscope and micro-EIT. When the sample started freezing, the brightness of the sample became higher and the relative impedance in micro-EIT image also increased. Similar results were obtained in all the four trials respectively. Therefore, these results indicate that EIT is useful to observe freezing process of a cell.

• 公益社団法人 計測自動制御学会の論文

著者

• 氏平 政伸

  北里大学大学院医療系研究科

• 根武谷 吾

  北里大学大学院医療系研究科

• 平賀 琢也

  （医社）蒼紫会森下記念病院

関連論文

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• 曲率センサの胸郭上配置を想定した胸郭形状推定法:—ウェアラブル電気インピーダンストモグラフィの一機能として—
• 電気インピーダンストモグラフィ（EIT）を用いた細胞凍結過程の可視化に関する基礎的検討

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