Interaction-dependent Native Chemical Ligation and Enzyme Reconstitution for Detection of Peptide–Peptide Interaction

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概要

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• A new detection method for ligand–protein interactions was constructed using interaction-dependent native chemical ligation and enzyme reconstitution (IDNCL-ER) by employing ribonuclease S. Using a coiled-coil peptide pair as a model of ligand–protein interactions, we determined that IDNCL-ER can work well to detect the peptide–peptide interactions by enzymatic activity.

• 公益社団法人 日本化学会の論文

著者

• Takahashi Tsuyoshi

  Advanced Scientific Research Leaders Development Unit Gunma University

• Saito Akinori

  Division of Molecular Science, Faculty of Science and Technology, Gunma University

関連論文

• A Novel Peptide Array Using a Phage Display System for Protein Detection
• Cell-adhesive hydrogels composed of peptide nanofibers responsive to biological ions
• Interaction-dependent Native Chemical Ligation and Enzyme Reconstitution for Detection of Peptide–Peptide Interaction

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