Expression and Activation of Mitogen-activated Protein Kinases in Matured Porcine Oocytes under Thermal Stress

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概要

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• In this study, we determined the expression and activation of p38 MAPK in matured porcine oocytes subjected to heat shock (HS). When MII oocytes were heated, only the phosphorylated p38 levels relative to the total p38 levels decreased (P<0.01) after HS, but no clear relationship with HS treatments was observed in the ERK, JNK and p90rsk expressions of matured oocytes. To confirm p38 activation in matured oocytes, immunocytochemical staining was performed to localize its expression and distribution in the ooplasm, and the results were largely consistent with previous Western blot analyses. Moreover, when matured oocytes were co-cultured with a P38 MAPK inhibitor, SB203580, for 4 h at 41.5 C, the activation of its immediate downstream substrate MAPKAPK-2 was not inhibited within any of the treatment groups. It appears that the MAPKAPK2 levels increased only under prolonged culture (HS4 h and C4h) compared with the control group. In conclusion, p38 activity in porcine oocytes was decreased after exposure to HS and prolonged culture. These alterations of p38 and activation of MAPKAPK2 may be associated with porcine oocyte viability under HS conditions, and a potential cross-talk between p38 MAPK and other signaling cascades may exist, which warrants additional investigation.

• 日本繁殖生物学会の論文

著者

• Ju Jyh-cherng

  Department Of Animal Science University Of Connecticut

• JU Jyh-Cherng

  Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC

• YEN Shih-Ying

  Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC

• TSENG Jung-Kai

  Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC

• CHUANG Show-Mei

  Institute of Biomedical Sciences, National Chung Hsing University, Taichung 402, Taiwan, ROC

• CHEN Shuen-Ei

  Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan, ROC

関連論文

• Surface Ultrastructural Characteristics of Bovine Oocytes Following Heat Shock
• Ascorbic Acid Improves the Developmental Competence of Porcine Oocytes After Parthenogenetic Activation and Somatic Cell Nuclear Transplantation
• Expression and Activation of Mitogen-activated Protein Kinases in Matured Porcine Oocytes under Thermal Stress

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