Effects of dynamics of water molecules at hydrophilic polymer brush surfaces on protein adsorption behavior:

概要

論文の詳細を見る
The suppression of protein adsorption must be required for a surface of biomaterials to avoid undesirable biological reactions. Understanding the interactions between proteins and surfaces is necessary to construct new biomaterials that have ultimate nonbiofouling property. Protein adsorption causes in an aqueous medium, therefore, we focused on the hydration state at the interface of the aqueous medium with proteins and the contacting materials, and investigated the effects of hydration state on protein adsorption behavior towards the surface. We could successfully establish the method for evaluation of dynamics of water molecules in the vicinity of the surface by using proton nuclear magnetic resonance spectroscopy for the hydrated polymer brush layer-modified micro-silica beads. The zwitterionic and cationic polymer brush surfaces were used for the measurement. The results clearly indicated that the dynamics of water molecules determined protein adsorption onto the surfaces. Thus, the polymer brush surfaces with hydration layer and high-diffusion of water molecules in the layer, such as phosphorylcholine group-bearing polymer brush surface, were effective to inhibit protein adsorption.
一般社団法人日本MRSの論文