Staining of Semithin Tissue Sections Embedded in HPMA, Quetol 523 and MMA
スポンサーリンク
概要
- 論文の詳細を見る
Various tissues fixed in a mixture of formaldehyde and glutaraldehyde, and embedded in an improved 2hydroxypropyl methacrylate mixture were employed for studying the fine structures of cells and tissues by light microscopy. The embedding mixture contained Quetol 523 and methyl methacrylate as a plasticizer without a cross-linker. The catalyst was QCU-1. The mixture had a low viscosity, was easy to handle and penetrated readily and completely into the specimen, producing a homogeneous block from which it was easy to cut sections of 1-2μm in thickness. A wide variety of stains have been employed with such sections and those reported here are hematoxylin-eosin, Azan and PAS. There was excellent preservation of alkaline phosphatase activity. A method of poststaining immunoperoxidase labeling was also applied to the mouse pancreas and examples of staining with insulin are included.
- Editorial Board of Okajimas Folia Anatomica Japonicaの論文
Editorial Board of Okajimas Folia Anatomica Japonica | 論文
- Beiträge zur Anatomie des Lymphgefässsystems der Wirbeltiere und des Menschen (Japaner).
- Beiträge zur Anatomie des Lymphgefässsystems der Wirbeltiere und des Menschen (Japaner).
- Untersuchung über die periphere Bahn des N. vagus.
- Über den Golgi=Apparat in den Schilddrüsenzellen.
- Beiträge zur Histologie der Amphibienmilz, mit besonderer Beruüksichtigung auf ihre jahreszeitlichen Veränderungen.