Visualization of Mitochondria and Some Other Cytoplasmic Structures in Hematoxylin-eosin Stained Sections by Fluorescence Microscopy
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Summary: In hematoxylin (H)-eosin (E) stained, paraffin sections prepared after fixation in 10% neutral buffered formalin, fluorescence microscopy demonstrated mitochondria, juxtaglomerular granules and some other structures in various tissues. Numerous fluorescent mitochondria were observable in hepatocytes under violet or green excitation. In hydrazine-fed mice, mitochondria in hepatocytes were large. In pancreatic acinar cells, bright filamentous mitochondria were demonstrated in the basal cytoplasm and intensely fluorescent secretory granules were in the luminal cytoplasm. Parietal cells of the stomach showed densely packed, fluorescent granular mitochondria. In epithelial cells of the kidney, rod-shaped fluorescent mitchondria exhibited a distinct striation in the basal cytoplasm. Juxtaglomerular cells in the afferent arteriole contained secretory granules which were more intensely fluorescent than mitochondria. Lipofuscin granules were autofluorescent in H-E stained sections of the ovarian interstitial cells, testicular Leydig cells and adrenal cortical cells.
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Editorial Board of Okajimas Folia Anatomica Japonica | 論文
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