Immunocytochemical Localization of D-Amino Acid Oxidase in the Proximal Tubule Cell of Porcine Kidney
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The immunocytochemical localization of D-amino acid oxidase was demonstrated in the proximal tubule cells of porcine kidney. D-amino acid oxidase was localized predominantly on the cell membrane of the brush border which could be seen on the free surface of each cell. On the inner-membrane of the apical canaliculi and large vacuoles, reaction product showing the localization of D-amino acid oxidase was demonstrated. The positive reaction was also recognized on the plasma membrane of the lateral cell surfaces and the basal infoldings. It was suggested that D-amino acid oxidase was localized on the surface of cytoplasmic membrane which seemed to have a high activity of resorption of amino acids and various substances.
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