Modified Technique of Mesulam's Tetramethylbenzidine Method to Prevent Fading of the Color of the Reaction Product
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概要
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Trials to prevent fading of the color of tetramethylbenzidine reaction product were performed after injection of horseradish peroxidase (HRP) into the vitreous body of albino rats. Sections mounted on slides were dried immediately after the end of the HRP histochemical reaction and soaked directly in chilled xylene. These procedures were performed in a dark room and the preparations were stored in a refrigerator. By means of this technique, almost all of the HRP positive fibers were observable even after 14 months of storage.
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