Histochemical Observation of GMA-Quetol 523 Embedded Tissue by Light Microscopy
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概要
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The use of GMA-Quetol 523 mixtures as an embedding medium permits the cutting of 1 to 2μm sections from polymerized blocks. Quetol 523 reacts with GMA and becomes an integral part of the polymerized system, and the mixtures have an innocuous chemical behavior for histochemistry. The mixtures can be polymerized at both 60°C and 39°C. Tissue embedding at 39°C can thus be used to avoid loss of enzyme activity. This offers considerable advantages in enzyme histochemistry. Light microscopic examinations of mouse tissues fixed in Bouin's, Gendre's, Zenker's, Carnoy's, Ciacio's fixatives, acetone and some aldehydes (glutaraldehyde, paraformaldehyde and acrolein) have revealed clear morphology with little distotion and shrinkage. Enzyme activities (alkaline phosphatase, acid phosphatase and nonspecific esterase) of tissues fixed in glutaraldehyde, calcium-formalin, acrolein and absolute acetone have been preserved without loss of staining specificity and localized far more precisely than in paraffin or frozen sections. GMA-Quetol 523 mixtures thus promise to be very useful embedding media for use in histochemistry.
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