The Preservation of Fine Structure by Use of Dimethylsuberimidate as a Fixative
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概要
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The fixation effects of d i methylsuberimidate (DMS) and other diimidoesters, which react with amino groups of proteins and cause the intra- and intermoleculer cross-links of proteins, were investigated, in order to develop the fixative which is expected to be suitable for the histochemical study. Before applying these reagents to histochemical study, a comparison was made with glutaraldehyde about the preservation of fine structures of various organs. The ultrastructure of liver, kidney, small intestine, lung, pancreas, striated muscle, cardiac muscle and testis was preserved very well after the fixation with DMS, and did not show much noticeable differences from that with glutaraldehyde fixation. Since DMS worked very well from the morphological standpoint, and since DMS is known to cross-link the proteins safely from the biological data, this reagent is now ready for tentative application for histochemistry as a fixative.
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