Sudan Black B Stain with Paraffin Sections in Early Tooth Germ
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(1) With Sudan black B stain lipid was a bluish tint in the deparaffinized sections, and was caesious in color in the non-deparaffinized sections. The cells and tissues, where lipid was localized, were approximately the same in both sections, and their distribution corresponded with the distribution in the frozen sections.<BR>In the bud stage lipid was present in the buldged part of the dental epithelium, and in the cap stage it was recognized in the inner and the outer enamel epithelium, in the boundaries between the inner and the outer enamel epithelium, and in the enamel knot. Lipid was hardly recognized in the stellate reticulum and in the dental papilla.<BR>(2) In the deparaffinized sections, the transparent droplet-like or little crystalline substances (2-3μ ) were situated, slightly in the inner part, and remarkably in the buidge and in the free end of the dental epithelium in the bud stage. In the cap stage they were recognized in the dentale Pithelium, especially in the enamel knot. They were slightly recognized in the oral epithelium, but they were not in the dental papilla and in the surrounding tissues of the tooth germ. In the non-deparaffinized sections; The droplet-like or little crystalline substances (2-3μ ) were remarkably recognized in the oral epithelium. They were scattered in the dental epithelium, unlike the deparaffinized sections, they were not abundant in the swollen part and in the free end of the bud stage, and they were not remarkable in the enamel knot of the cap stage. In the surrounding tissues of the tooth germ, including dental sac, they were situated as a group at a certain dis- tance from the margin of the tooth germ on the buccal side, or were observed along the dental sac, describing a circular arc.<BR>They were not recognized in the frozen sections. It seems that they are neutral fat or cholesterin crystal.<BR>(3) In the non-deparaffinized sections, the streak-like or the embossed carving figures were seen in the dental epithelium, especially they were often recognized in the free end and in the buldge of the bud stage, and in the inner enamel epithelium and in the circumferences of the enamel knot of the cap stage. It is assumed that they indicate the position of the invagination in the dental epithelium.<BR>(4) Some specific spindle cells in the dental epithelium and some irregular-shaped mesenchymal cells in the dental papilla contiguous to the dental epithelium were stained more bluish or Nile green in color than any other cells in the tooth germ, and some of them were not stained except the nucleolus. They were often recognized in the periphery of the streak-like or the embossed carving figures in the dental epithelium of the non-deparaffinized sections.<BR>(5) It is thought in the paraffin sections that post-chroming stain with Kernecht-Rot is difficult.
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