Isolation and Differentiation of Human Adipose-Derived:Stem Cells into Adipose, Bone, and Cartilage Cells
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概要
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Recently, human adipose-derived stem cells (ASCs) h ave been discovered and reported to show the same multilineage potential as bone marrow mesenchymal stem cells. In this study, we observed normal human adipose tissue using a scanning electron microscope, and induced the differentiation of ASCs isolated from abdominal subcutaneous tissue into adipose, bone, and cartilage cells. Subsequently, we induced the differentiation of ASCs seeded on hydroxyapatite into osteocytes in an attempt to promote the formation of bone with a three-dimensional morphology. We succeeded in preparing three-dimensionally cultured bone using human ASCs in the subcutaneous tissue of nude mice. It is revealed that collagen scaffolds are useful for human ASCs as a three-dimensional bone tissue engineering scaffold <I>in vitro</I> and <I>in vivo</I>. Various staining techniques demonstrated that ASCs were able to differentiate into mature adipose, bone, and cartilage cells, suggesting that adipose tissue is a useful source of mesenchymal stem cells in tissue engineering.
- The Medical Society of Kansai Medical Universityの論文