Comparison of relative DNA content estimated using DAPI and PI-FCM in Miscanthus sinensis , Miscanthus sacchariflorus , and their hybrids

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Three triploid Miscanthus hybrids were found in Kushima, Japan, by a first screening of M. sinensis and M. sacchariflorus plants by using 4',6-diamidino-2-phenylindole, dilactate dye flow cytometry (DAPI-FCM). The ploidy levels of the three triploid hybrids were confirmed by a second screening using propidium iodide dye flow cytometry (PI-FCM). It was possible that we might have missed a few triploid hybrids in the first screening because of a weak correlation of relative estimated DNA contents between PIFCM and DAPI-FCM. Therefore, to get the conclusive evidence of three accessions newly discovered being triploid among some Miscanthus spp., we conducted a comparison of the relative DNA contents estimated with AT-selective DAPI dye and intercalary PI dye in diploid (2x) M. sinensis and tetraploid (4x) M. sacchariflorus and their triploid (3x) hybrids. A strong linear correlation was observed between both FCM analyses. The slope of the regression line was 1.15. This result shows the high reliability of the findings of our previous study on Miscanthus accessions.
日本暖地畜産学会の論文