Development of a Fluorescent Microscope Combined with a Real-time Autoradiography System

概要

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For combination with microscope, we developed real-time autoradiography system for micro-scale analysis with adjustment of the CsI(Ti) scintillator thickness for higher resolution and applying tapered fiber optic plate for magnification of autoradiograph image. We combined real-time autoradiography system with an inverted fluorescent microscope so that an autoradiograph image as well as fluorescent image, bright-field image can be acquired at the same time. In the case of observation of sliced soybean stalk traced <SUP>45</SUP>CaCl, the fluorescent and bright-field image was acquired which magnified to 50 times, the autoradiograph image of <SUP>45</SUP>Ca distribution in the tissue was acquired in almost same scale. The new microscopic system which can acquire autoradiograph image of labeled signals (low molecular weight) is expected to develop the signal transduction study and gene expression, combined with fluorescent protein techniques such as GFP etc.
公益社団法人日本アイソトープ協会の論文