か粒球系細胞に対する単クローン抗体の作製およびそれらの各種白血病との反応特異性
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概要
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A panel of monoclonal antibodies reactive with myeloid cells at different stages of developement was produced and was used to characterize the leukemic cells of 62 patients. Monoclonal antibodies included TG8/TG28 (anti-granulocytes), TM2 (anti-platelets, granulocytes, monocytes and cALL), TM15 (anti-monocytes, granulocytes and null cells), TM18 (anti-monocytes), TM1.30 (anti-monocytes and extra), TM2.7 (anti-cell lines?), TP80 (anti-platelet glycoprotein IIb/IIIa complex), TP82 (anti-cALL associated antigen, p 24), NC1 (anti-HLA-DR framework), and NC6 (anti-HLA-ABC framework).TG8/TG28 were reactive with not only mature granulocytes but also cells from some AML, APL and AMML. TM2 reacted with leukemic cells from patients with AML, APL, AMML, EL, CML in chronic phase and myeloid blastic crisis and also common ALL. TM15 reactive cells were observed in 6/11 cases of AML, 2/3 cases of APL, 3/3 cases of AMoL or AMML but none of T or B cell malignancies. However, TM18 had narrower specificity against AML (4/11), APL (2/3), AMoL or AMML (2/3) and no positive cells were present in lymphoid malignancies. Common ALL-associated antigen (p24) recognized by TP82, was shown to be expressed on platelets, cALL and on some other acute lymphoblastic or myelo (mono) blastic leukemias. NC6 confirmed the expression of major histocompatibility complex class I antigen on lymphoid, myeloid and erythroid leukemias. TM2.7 reacted with only a part of cell lines but not with any leukemic cells. No definitive reactivity pattern of TM1.30 was observed against leukemic cells or cell lines.Cells from all patients with CML in chronic phase were reactive with TG8/TG28 and TM15 with more than 50% in reactivity but cells bearing these antigens were decreased in myeloid blastic crisis. In contrast, blast cells gave abundant expression of HLA-DR antigen. Three out of eight cases with myeloid crisis had TP80 positive cells in their blood were observed. This result suggested that megakaryocytic lineage cells might take more part in myeloid crisis than has been expected. It was shown that HLA-DR antigen, that was recognized by NC1, was more abundantly expressed on blast cells in crisis than on cells in chronic phase.
- 一般社団法人 日本血液学会の論文
一般社団法人 日本血液学会 | 論文
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