Attempts at Organ-specific In Vivo Short-term Tests for Environmental Mutagens and Carcinogens in Rodent Liver and Stomach
スポンサーリンク
概要
- 論文の詳細を見る
The primary motivation for conducting short-term tests for environmental mutagens and carcinogens has been to predict mutagens and/or carcinogens and to assess any associated risks. Organ-specific in vivo short-term tests in rodents are valuable because chemical carcinogenesis is generally organ-specific. I have attempted to develop various organ-specific in vivo short-term tests mainly in rodent liver and stomach. Recently, our collaborative study group, Toxicogenomics/Japanese Environmental Mutagen Society·Mammalian Mutagenicity Study Group (JEMS·MMS), attempted to use gene expression profiling in in vivo short-term tests, conducted DNA microarrays to extract candidate marker genes, and later shifted to quantitative real-time PCR (qPCR) to profile the expression of selected genes. We successfully discriminated 8 genotoxic hepatocarcinogens from 4 non-genotoxic hepatocarcinogens by statistical analysis using principal component analysis (PCA) based on the gene expression profiles for 12 genes (Aen, Bax, Btg2, Ccnf, Ccng1, Cdkn1a, Gdf15, Lrp1, Mbd1, Phlda3, Plk2, and Tubb2c) in mouse liver at 4 and 48 h following a single intraperitoneal administration of chemicals as determined by qPCR. More recently, we successfully performed a similar study in rat liver. Previously, my collaborators and I developed various organ-specific in vivo short-term test methods, including UDS (unscheduled DNA synthesis); RDS (replicative DNA synthesis) using a liquid scintillation counter in rat glandular stomach, forestomach, colon, and liver and hairless mouse epidermis; DNA single-strand scission (DSS); and ornithine decarboxylase assay (ODC) in rat glandular stomach on 62 compounds. Developing short-term tests that are helpful for the risk assessment of human mutagens and carcinogens would contribute to the development of ideal prediction methods.
- 日本環境変異原学会の論文
日本環境変異原学会 | 論文
- P-145 化学物質の変異原性に関するEU分類とドイツMAK分類の比較(ポスターセッション)
- P144 生殖細胞変異原のGHS分類基準適用における問題点
- P-98 GHSにおける生殖細胞変異原性物質の分類(ポスター(2))
- リスクアセスメントにおける遺伝毒性 : 海外の動向と視点(第33回大会シンポジウム1「発がん性と遺伝毒性の閾値 : リスクアセスメントにおける問題点」)
- S2-4 環境汚染物質による野生生物のリスクを評価するための科学的方法(シンポジウム2:「環境と食の安全性にサイエンスが果たす役割」)