Analyses of 8-Methyldeoxyadenosine and 8-Methyldeoxyguanosine as Markers of Free Radical-mediated DNA Methylation in Mouse
スポンサーリンク
概要
- 論文の詳細を見る
To examine whether methyl radical-induced DNA damage occurs in vivo, cumene hydroperoxide (CuOOH) and methionine sulfoxide (MetO) were administered to mice as methyl radical generators, and 8-methyldeoxyadenosine (m8dA) and 8-methyldeoxyguanosine (m8dG) in the tissue DNA were analyzed by the LC/MS/MS method. After mouse skin was treated with CuOOH and Fe2+, both m8dA and m8dG were detected in the epidermal DNA. m8dA formation was also detected in the liver DNA from MetO-treated, non-alcoholic steatohepatitis (NASH) mice. Since 5-methyldeoxycytidine, m8dA, and m8dG are formed in DNA by methyl radicals, these results support our hypothesis that de novo DNA methylation at the cytosine C-5 position is triggered by a free radical mechanism and may initiate epigenetic changes.
- 日本環境変異原学会の論文
日本環境変異原学会 | 論文
- P-145 化学物質の変異原性に関するEU分類とドイツMAK分類の比較(ポスターセッション)
- P144 生殖細胞変異原のGHS分類基準適用における問題点
- P-98 GHSにおける生殖細胞変異原性物質の分類(ポスター(2))
- リスクアセスメントにおける遺伝毒性 : 海外の動向と視点(第33回大会シンポジウム1「発がん性と遺伝毒性の閾値 : リスクアセスメントにおける問題点」)
- S2-4 環境汚染物質による野生生物のリスクを評価するための科学的方法(シンポジウム2:「環境と食の安全性にサイエンスが果たす役割」)