Social Stress Induces Oxidative DNA Damage in Mouse Peripheral Blood Cells
スポンサーリンク
概要
- 論文の詳細を見る
We investigated whether or not social stress (isolation, 1 mouse per cage) increases oxidative DNA damage in mouse peripheral blood cells. Male BALB/c mice (4 weeks old) were housed 5 per cage for 10 days. After acclimatization, mice were exposed to isolation stress for 7 and 30 days. Control mice were housed 5 per cage. Serum levels of corticosterone, which is a well known stress marker, and antioxidant compounds, ascorbic acid and α-tocopherol, were determined by liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) and ESACoul Array analysis, respectively. Single cell gel electrophoresis (comet assay) using formamidopyrimidine DNA glycosylase (FPG) was done to determine oxidative DNA damage in mouse peripheral blood cells. The significant increases of plasma level of corticosterone were observed in mice exposed to isolation stress for 7 days and 30 days. Although no significant differences in plasma concentration of ascorbic acid and α-tocopherol were observed between the control mice and the isolated mice, oxidative DNA damage was induced in the isolated mice for 7 days and 30 days. These results suggest that social stress, isolation, causes mild oxidation in mice.
- 日本環境変異原学会の論文
日本環境変異原学会 | 論文
- P-145 化学物質の変異原性に関するEU分類とドイツMAK分類の比較(ポスターセッション)
- P144 生殖細胞変異原のGHS分類基準適用における問題点
- P-98 GHSにおける生殖細胞変異原性物質の分類(ポスター(2))
- リスクアセスメントにおける遺伝毒性 : 海外の動向と視点(第33回大会シンポジウム1「発がん性と遺伝毒性の閾値 : リスクアセスメントにおける問題点」)
- S2-4 環境汚染物質による野生生物のリスクを評価するための科学的方法(シンポジウム2:「環境と食の安全性にサイエンスが果たす役割」)