Viability of the Cariostat medium as a source of DNA for further analysis through polymerase chain reaction
スポンサーリンク
概要
- 論文の詳細を見る
This simulation study was carried out to analyze and evaluate Cariostat-inoculated samples through a conventional PCR protocol specific for <I>Streptococcus mutans</I> strains and also for the purpose of establishing acceptable storage conditions for Cariostat-inoculated samples in cases in which optimal storage conditions are not feasible. A reference strain of <I>Streptococcus mutans</I> (ATCC 25175) was used in the study. The samples were subjected to different inoculation, incubation and storage conditions, and bacterial viability was checked through a conventional PCR technique and by assessment of colony growth on different agar media. Band detection of all samples incubated for even up to 120 hours at 37°C was still possible, indicating less DNA degradation. Samples stored at -20°C yielded results closest to those of samples incubated normally (without storage), followed by samples stored at 4°C and then samples stored at room temperature. The difference in these results, however did not influence the quality of DNA to an unsatisfactory level. For bacterial viability through agar plating, even if there were no more viable growth on the plates, band detection from strains inoculated and stored in Cariostat was still possible. From these results, we conclude that samples stored under various conditions can be further analyzed using DNA extraction protocols for conventional qualitative PCR amplification.
- 一般社団法人 日本小児歯科学会の論文
一般社団法人 日本小児歯科学会 | 論文
- Dental caries experience among kindergarten school going children of, India
- Estimation of nasal tip position using lateral cephalometric X-ray images in Japanese male children: Applications in facial reconstruction
- The characteristics of the deciduous dental arch form of children in Changchum-city, China
- Presence of Streptococcus mutans or Streptococcus sobrinus in Cariostat-inoculated plaque samples from Japanese mother-child pairs
- Viability of the Cariostat medium as a source of DNA for further analysis through polymerase chain reaction