The Effects of Dibutyltin (DBT) Dichloride on the Viability and the Productions of Tumor Necrosis Factor .ALPHA. and Interleukin-12 in Murine Macrophage Cell Line, J774.1.
スポンサーリンク
概要
- 論文の詳細を見る
Immunotoxicity is one of toxic effects of dibutyltin(DBT)compounds. In our previous study, the inhibitory effects of DBT on the productions of tumor necrosis a(TNFα)and interleukin-1β in a murine macrophage cell line, J774.1, were demonstrated. In this study, the effects of DBT dichloride from 0.125 µM to 2.0 µM on the cell viability and productions of cytokines including TNFα, IL-10 and IL-12p40 were investigated. J774.1 cells were exposed to DBT dichloride at 0, 0.125, 0.25, 0.5, 1.0, 1.5 or 2.0 µM. After 18 hours, lipopolysaccharide was added to each well. Total RNA was extracted from the cells after an additional 6 hours of incubation. Real-time PCR was used to analyze the mRNA expression for TNFα, IL-10, IL-12p40 and glycelaldehyde-3-phosphate dehydrogenase(GAPDH)in J774.1 cells. The cell viabilities were determined and the supernatants were sampled after additional 24 hours of incubation. The concentrations of TNFα, IL-10 and IL-12p40 in the supernatant were determined by ELISA. The mean cell viabilities in the groups exposed to DBT at 0.5 µM and over were significantly lower than that of the control. The mean mRNA expression of IL-12p40 in the 0.25 µM group was significantly higher than that in the control group. The mean concentrations of TNFα in the supernatant in the 1.0, 1.5 and 2.0 µM groups were significantly lower than that of the control, and that in 0.125, 0.25 and 0.5 µM groups were significantly higher. The mean concentration of IL-12p40 in the 0.25 µM group was significantly higher than that of the control and that in the 1.0, 1.5 and 2.0 µM groups were significantly lower. There were no significant differences in mRNA expression and protein level for IL-10. DBT is toxic to macrophages, and the inhibition of the production of cytokines by DBT does not occur for all cytokines.
- 日本微量元素学会の論文
日本微量元素学会 | 論文
- 微量元素と老化 (特集:微量元素と健康)
- ヒジキに含まれるヒ素の健康リスク評価
- Relationship between Genetic Polymorphism of Arsenic (+3 oxidation state) Methyltransferase (AS3MT) and Profile of Urinary Arsenic Compounds in Vietnamese
- Accumulation Features of Arsenic in Hawksbill Turtles and Green Turtles
- Arsenic pollution in groundwater of Vietnam and Cambodia: a review (特集 臨床における亜鉛の有効性の探索)