Molecular Cytogenetic Analysis of the Critically Endangered Trigonotis radicans var. radicans and var. sericea and Allied Species in Japan

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• Chromosome numbers of four Trigonotis taxa, T. brevipes (2n=24), T. peduncularis (2n=54), T. radicans var. radicans (2n=28), and T. radicans var. sericea (2n=24), were investigated and reported here for the first time. Our findings suggest that the chromosome number of T. radicans var. radicans represents 2n=24+4, because four small chromosomes were distinguished among the total of 28. FISH techniques revealed 5S rDNA sites in different chromosomes with 18S rDNA sites in T. brevipes and T. peduncularis, but in the same chromosomes in the two varieties of T. radicans. Phylogenetic analysis based on the ITS1-5.8S-ITS2 sequences further suggested that the chromosomal features of T. radicans are specific and perhaps derived from within the genus.

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財団法人 日本メンデル協会 | 論文

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