Karyotype and RAPD Analysis in 3 Commercial Varieties of Cucurbita maxima Duch.
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概要
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Three commercial varieties of Cucurbita maxima Duch. (Sweet Gourd or Pumpkin) such as Baromashi (Local), Solid Gold and BARI Mistikumra-2 were investigated karyotypecally and at molecular level by RAPD analysis for authentic characterization. Three varieties were found to possess 2n=45–48, very small chromosomes ranging from 0.2–1.0 μm in length. The chromosomes of Baromashi (Local) and BARI Mistikumra-2 could be grouped in 4 classes on the basis of chromosomal length whereas the Solid Gold variety has only 3 such classes. The smallest chromosomes of 3 varieties were so minute that each karyotype showed at least 2 sharp modalities. Each variety showed distinct RAPD fingerprinting with 7 different primer combinations. The variety Baromashi (Local) and BARI Mistikumra-2 showed exactly similar banding pattern in primer OPA-3 and OPA-6. Maximum common band was found in OPD-3 suggesting the sharing of similar sized fragments among these 3 varieties. No common band was found in OPA-6 indicating the highest polymorphism (100%). The average polymorphism among these 3 varieties in different primer combinations was 64.35%. Solid Gold showed 8 unique bands in different sizes whereas Baromashi (Local) and BARI Mistikumra-2 had 7 and 5 unique bands, respectively. Karyotype and RAPD fingerprinting were almost similar in Baromashi (local) and BARI Mistikumra-2 whereas Solid Gold completely different from the other 2 in these parameters. These results were supported by different phenotypic or morphological features. Therefore, with the help of karyotype and RAPD analysis, it was possible to characterize 3 varieties of Cucurbita maxima Duch.
- 財団法人 日本メンデル協会の論文
財団法人 日本メンデル協会 | 論文
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