RAPD and Cytogenetic Study in F1 and F2 of Interspecific Cross between Gossypium arboreum*Gossypium anomalum
スポンサーリンク
概要
- 論文の詳細を見る
An interspecific hybrid between G. arboreum×G. anomalum was obtained by pollinating previous day bagged flowers of G. arboreum genetic male sterile line MPKV GMS with pollen from wild G. anomalum.The chromosome constitution in all the plants of G. arboreum and G. anomalum were found to be normal while interspecific hybrid showed mean chromosome association 1.081I+10.479II+0.000III+0.990IV with normal sporads. Interspecific hybrid showed anomalous behavior of univalent and formation of single and double chromatin bridges both during fist and second meiotic division of meiosis. Meiotic chromosome association was observed in nine plants of F2 generation. The cytological studies showed that F1 and F2 generations were having irregular pairing and unequal separation of chromosomes. This led to pollen sterility in these generations. In F2 plants some good plants identified with higher pollen fertility and more number of bolls per plant. Selections from these plants can help to introgress desired characters from G. anomalum to G. arboreum. Random Amplified Polymorphic DNA (RAPD) analysis was employed to characterize the F1 interspecific hybrid of G. arboreum MPKV GMS×G. anomalum and its nine segregants. A total fifteen primers of 10 mer were screened of which eight were selected based on polymorphism of amplified fragments, amplification intensity and the presence of informative markers. Maximum 98 amplified fragments were resolved on the gel for parents, F1 and F2's with OPC-19 primers, whereas, minimum 25 fragments were seen with OPD-04 primer. Similarity coefficient based on DNA amplification using amplified RAPD primer was estimated. The values for genetic similarity ranged from 0.14 to 0.77.
- 財団法人 日本メンデル協会の論文
財団法人 日本メンデル協会 | 論文
- The Chromosomes of Dicranocephalus agilis (Hemiptera: Heteroptera)
- Consistently greater decondensation of the nuclear DNA-protein complexes from normal lymphocytes than from acute and chronic lymphocytic leukemia cells following treatment with disulfide reducing agents.
- The Reversion of Chromoplasts to Chloroplasts in Valencia Oranges
- Two Problems in the Interpretation of Meiosis in Plants
- Cytology of Trichosanthes palmata Roxb