A Study of Nucleolus Organizing Regions by Ag-staining, in situ Hybridization and Chromomycin A3 Techniques in Tilapia rendalli (Cichlidae, Perciformes) of the River Iguacu Basin, Parana, Brazil

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• Chromosomes of Tilapia rendalli from river Iguaçu, particularly its nucleolus organizing regions, was analyzed by Ag-staining NOR (Ag-NOR), Chromomicyn A3 (CMA3) and fluorescence in situ hybridization techniques (FISH). Karyotypic analyses showed diploid number 2n=44, with 10 submetacentric (SM), 8 subtelocentric (ST) and 26 acrocentric (A) chromosomes, and constitutive pericentromeric heterochromatin standard for most chromosomes. Whereas Ag-NOR and CMA3 detected 2 chromosome pairs, FISH method revealed 3 chromosome pairs with fluorescent signs and, thus, additional 18S cistrons. Results show that additional rDNA sites was inactive. Whilst active pairs were heterochromatic and CMA3 positive, the inactive one was heterochromatic and CMA3 negative.

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財団法人 日本メンデル協会 | 論文

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