Profiles of Virulence-associated Genes of Avian Pathogenic Escherichia coli Isolates from Chickens with Colibacillosis
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概要
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The colibacillosis caused by avian pathogenic <I>E. coli</I> (APEC) is responsible for a significant loss of productivity and mortality in the poultry industry. The pathogenicity of these bacteria is based on the presence and expression of various virulence factors. In this study, the presence of the 19 virulence-associated genes in APEC was determined using PCR. Among the 118 <I>E. coli</I> isolates from the chickens with colibacillosis, all contained at least one of the 19 genes as approximately 95% of the isolates contained <I>fimC.</I> Interestingly, the <I>clpG</I> gene, which has not been detected in APEC previously, was detected in half of the isolates. The ColV plasmid-associated genes such as <I>colV, tsh, iucC, iucD and iss</I> genes were also detected in 57.6, 55.9, 50.0, 47.5, 47.5 and 41.5% of isolates, respectively. With regard to the fimbrial genes, the <I>papA</I> (14.4%), papC (14.4%) and <I>papG</I> genes (15.2%) were identified at relatively low rates, none of the isolates harbored <I>afa8D, f17A</I> or <I>facA</I>, and only 3 of the isolates (2.5%) contained <I>eaeA.</I> In this study, 94 isolates harbored two or more of the genes, and there were 43 different patterns of gene combination in the isolates. The most common pattern, which was found in 14.4% (17 isolates), was <I>clpG-fimC-iutA-colV-tsh-iucC-iucD-irp2-fyuA-vat-iss.</I> Overall, these results suggest that APEC strains in this area commonly contain multiple virulence factors and approximately half of the APEC strains contained the ColV plasmid-associated genes. Especially, <I>colV</I> and <I>tsh</I> were detected more than half of the isoaltes.
- 日本家禽学会の論文
日本家禽学会 | 論文
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