組織Plasminogen Activatorの研究:慢性副鼻腔炎粘膜における特徴
スポンサーリンク
概要
- 論文の詳細を見る
In inflammation, the plasmin, one of some chemical mediators, has an important role. That is to say, because of the proteolytic activity of plasmin, it can produce the other chemical mediators during various inflammatory responses. The precursor of plasmin, plasminogen was converted to plasmin by the plasminogen activator which existed in various tissues and body fluids. However, during the inflammatory process the plasminogen activator derived from the infiltrated leukocytes and macrophage might be closely related to activate the plasminogen.<BR>In order to clarify the role of tissue plasminogen activator in inflammatory process, a comparative study was done on the chemical properties of the plasminogen activator from the pig heart and human paranasal mucous membrane with chronic sinusitis. These results obtained from this experiment were as follows:<BR>1) The preparation from the pig heart exhibited relative thermal stability at neutral pH, the molecular weight similar to that of ovalbumin, about 46,000, estimated by Sephadex G-150 gel filtration.<BR>2) The preparation from the paranasal mucous membrane exhibited two peaks of fibrinolytic activity by means of Sephadex G-200 gel filtration, indicating the existence of two different activators (high and low molecular weight). This tissue plasminogen activator with low molecular weight were very thermolabile at neutral pH, and the molecular weight was lower than that of cytochrome C (12,000).<BR>3) From these results it was suggested that this tissue plasminogen activator with low molecular weight might be produced by the other protease which existed in the locus of the chronic inflammation. In analogy with staphylokinase in vitro the proteolytic modification of tissue plasminogen activator might occur at the locus of chronic sinusitis.
- 耳鼻と臨床会の論文