Studies on Human Proinsulin C-peptide Radioimmunoassay Method:—Preparation of Antiserum and Its Specificity—

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The antisera using at final dilution of 1 : 10, 000 have been prepared by immunizing synthetic human proinsulin connecting peptide to rabbits for human proinsulin C-peptide radioimmunoassay. The cross reactivities of human proinsulin C-peptide derivatives with the prepared antisera were reduced by leaving amino acid residues from N terminal, although this phenomenon was a little different among antisera. Those results suggested that main antigen determinant in N terminal 31-38 of human proinsulin connecting peptide. The cross reactivities of other animal proinsulin C-peptide and other peptide hormones with the prepared antisera were not recognized at 103 p mole/ml.
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