ACTIONS OF ARACHIDONIC ACID METABOLITES IN THE CELLS RECOVERED FROM BRONCHO-ALVEOLAR LAVAGE FLUID AGAINST ANTIGEN INDUCED AIRWAY CONTRACTION IN GUINEA PIGS
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Stimulative or inhibitory actions of arachidonic acid (AA) metabolites released from CB (cells recovered from broncho-alveolar lavage fluid) against antigen-induced contraction of guinea pig trachea were examined. Tracheal strips were stimulated with ovalbumin (OA) or OA+A23187 in the presence or absence of CB. Tracheal strips were deviled into 4 groups, depending on the difference of stimuli, (1) OA, (2) OA+A23187, (3) CB+OA, (4) CB+OA+A23187. Contraction of strips was expressed as a ratio to that induced by acetylcholine at 10-4M.Contractions induccd by OA, OA+A23187, CB+OA and CB+OA+A23187 were 1.11±0.05, 1.27±0.32, 1.43±0.20 and 0.55±0.15, respectively. when compared with that induced by OA, contractions induced by CB+OA+A23187 were reduced significantly. Contraction induced by CB+OA seemed to be enhanced, but not significantly. TXB2 released into the incubation medium was measured by RIA and the concentrations in the groups of OA and OA+A23187 were lower than the detectable range. The concentrations in the groups of CB+OA and CB+OA+A23187 were 292 and 468pg/0.1ml, respectively.Concentrations of 6keto-prostaglandin (PG) F1α in the groups of CB+OA and CB+OA+A23187 were 29.00 and 39.25pg/0.1ml which were significantly higher than thet of OA. The results indicate that cells recovered from BALF, mainly macrophages, may play a regulatory role in the reaction of hypersensitivity of guinea pig trachea by synthesizing stimulative and inhibitory mediators of AA. TXA2 may play an important role as a contractive mediator. PGE2 and PGI2 were determined to be produced in small amounts, but may not play as important a role as dilative mediators because of their productivity.
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