Evaluation of Fluorescence Polarization Immunoassay Using Monoclonal Antibody for Determination of Cyclosporine in Whole Blood from Children Who Had Received Allogeneic Bone Marrow Transplantation.
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The fluorescence polarization immunoassay method using a monoclonal antibody (m-FPIA) was introduced for the determination of whole blood cyclosporine (CyA) concentration in 96 samples from six children who had received allogeneic bone marrow transplantation (BMT). When steady-state trough levels seemed to be achieved, eight blood samples in each patient were serially collected during 12 hours after intravenous infusion and an oral administration of CyA. CyA levels determined by the m-FPIA method were almost at the same levels (<I>r</I> =0.996, <I>p</I> <0.001) as those determined by the <SUP>125</SUP>I-labeled radioimmunoassay (m-RIA) method. There was a linear correlation [slope of 1.04; y intercept (m-FPIA values) =-19] between the m-FPIA and m-RIA methods. Since the m-FPIA method is faster and simpler than other methods, this assay will give us reproducible results for the routine monitoring of CyA in patients who undergo BMT.
- 特定非営利活動法人 日本小児血液・がん学会の論文
特定非営利活動法人 日本小児血液・がん学会 | 論文
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