Assessment of the Effect of Recombinant IL-3 by the Serum-Free Liquid Culture Method.
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In this paper we report on the effect of recombinant human IL-3 on CFU-GM in human umbilical cord blood progenitor cells in liquid culture. The study was conducted using the 2-step culture method. In the 1st, mononuclear cells, including adherent cells, were cultured under serumfree liquid culture conditions, dividing them into those to which rhIL-3 20 ng/ml was added and those to which it was not. We then isolated CFU-GM derived colonies in the presence of G-CSF 20 ng/ml. The CFU-GM derived colonies increased considerably more in the presence of rhIL-3 than in its absence. The CFU-GM derived colonies in 6 serially-tested specimens before and after serum-free liquid culture increased 1.46-2.84 times in response to rhIL-3. No significant difference in effect on the CFU-GM derived colony number increasing action was observed in a liquid culture system supplemented with 10% FBS. The results of these studies demonstrated that the CFU-GM derived colony increasing action is observed even in serum-free liquid cultures, and this appears to be a method enabling more accurate analysis of the effect of rhIL-3 on hematopoietic stem cells.
- 特定非営利活動法人 日本小児血液・がん学会の論文
特定非営利活動法人 日本小児血液・がん学会 | 論文
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