Analysis of Resistance-Related Gene Expression in Doxorubicin-Resistant Leukemia Cell Line by DNA Microarray.
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Drug resistance continues to be a serious problem in cancer therapy. To analyze the gene expression profile in doxorubicin-resistant human leukemia cell lines, we established a doxorubicin-resistant phenotype through continuous exposure of the suspension culture of K562 leukemia cells to increasing doxorubicin concentrations. The resistant cell line K562/ADM was more resistant to doxorubicin than the parent cells (K562/P) and also exhibited cross-resistance to vincristine. P-glycoprotein expression in the resistant cell line was significantly higher than the parent line. Using the microarray method to investigate the resistant cell line, we detected many unexpected differences in gene expression in comparison with the parent cell line. We also investigated the doxorubicin-induced variations in the gene expression profiles of K562 parent cells. Cyclooxygenase-1 and heat shock protein 90 kDa showed increased expression in both K562/ADM cells and 8-hour doxorubicin-treated K562/P cells when compared to expression levels in non-treated K562/P cells by microarray as well as northern analysis. We concluded that DNA microarray would be an effective tool to discover new drug resistance mechanisms.
- 特定非営利活動法人 日本小児血液・がん学会の論文
特定非営利活動法人 日本小児血液・がん学会 | 論文
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