Analysis of Fibrinolysis in Neonates Using Plasma Clot Lysis Assay with Plasminogen Activators.
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The <I>in vitro</I> fibrinolytic activities in the cord venous plasma of 30 full-term neonates without clinical problems were measured by clot lysis assay after adding plasminogen activators (PA), recombinant tissue plasminogen activator (r-tPA) or urokinase (UK). The fibrinolytic assay was performed in a microtiter plate to form clotting by mixing 25 pl of citrated plasma, 25μl of the mixture with CaCl<SUB>2</SUB> (f. c. 10mM) and human α-thrombin (f. c. 2 U/ml), 25μl of plasminogen activators, and 25, μl of saline. This was followed by measuring the absorbance at 405 nm and calculation of the fibrinolytic rate (% clot lysis). Furthermore, the effect of factor XIII (FXIII) on this method was analyzed by adding 25μl of purified FXIII instead of saline to the assay. The fibrinolytic rate increased with increases in the concentration of plasminogen activators and reaction time. The fibrinolytic rate of cord plasma was higher than that of adult plasma, at f.c. 0.3125μg/ml of r-tPA at 10 and 20 min of the reaction time. There was, however, no significant difference in the fibrinolytic rate between cord plasma and adult plasma after adding UK. The higher fibrinolytic rate of cord plasma after adding r-tPA was recovered by adding purified FXIII. These data suggest that neonatal hemostatic plague had a hyperfibrinolytic response to r-tPA in comparison with adult plasma, which may be due to the low concentration of FXIII that forms tightly to the cross-linked fibrin.
- 特定非営利活動法人 日本小児血液・がん学会の論文
特定非営利活動法人 日本小児血液・がん学会 | 論文
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