顆粒球コロニ-形成刺激因子(G-CSF)の感染防御効果に関する研究-2-好中球ス-パ-オキサイド産生能に対するpriming効果

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In present study, we have investigated superoxide (O-2) production from human neutrophils by recombinant human granulocyte colony-stimulating factor (G-CSF) using the microtiter plate for the purpose of being close to the inflammatory site. G-CSF by itself did not induce the release of O-2 in human neutrophil on either Fetal Bovine Serum (FBS)-coated plate or plate uncoated with FBS, even if neutrophils were exposed for maximum 3 hr. However, the optimal concentration of G-CSF (50ng/ml) was able to prime human neutrophils with enhance of O-2 release stimulated by the chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine (FMLP) from 10-6 to 10-8 M, but not by the non chemoattractant such as phorbol myristate acetate (PMA), concanavalin A, and ionomycin. These findings indicate that G-CSF might enhance bactericidal activity of neutrophils by priming them penetrating into the inflammatory site.
日本感染症学会の論文