インフルエンザ様患者からのウイルス分離法についての検討--MDCK凍結保存細胞を用いたインフルエンザウイルスの分離

概要

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To save labor and make it easy to take an immediate countermeasure at the time of isolation of influenza virus, studies were made on a method to inoculate a sample directly into cells which had been stored in a frozen state. The following results were obtained.<BR>1. When MDCK cells were stored at -80°C with DMSO as a low-temperature protecting agent, they showed a survival rate of more than 95% after storage for 6 months.<BR>2. MDCK cells stored in a frozen state presented a high susceptibility to the standard strain of influenza virus.<BR>3. When used for the isolation of virus from samples collected from patients, MDCK cells stored in a frozen state gave results not inferior to those obtained from monolayer culture cells.<BR>4. It was possible to take an immediate countermeasure when cells stored in a frozen state were used for the isolation of virus. Furthermore, the use of these cells could make it easy to isolate influenza virus even at a laboratory which was lacking in the equipment for cell culture and in experienced workers.
日本感染症学会の論文