acridine orange染色法による血液培養検体からの微生物迅速検出についての基礎的検討

概要

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The rapid detection of bacteremia in blood cultures is of great diagnostic and therapeutic importance. In this study, we evaluated a new visual detection method of microorganisms in blood culture, acridine orange (A. O.) staining technique, to use 5 commercial blood culture bottles and samples with 5%rabbit blood and 4 reference strains of aerobes. And the following results are obtained. 1. All culture bottles are macroscopically positive within 24 hrs of incubation.By A. O. staining technique, E. faecalis and E. coli can be detected at only 8 hrs in some culture bottles, but smears of S. aureus and P. aeruginosa are negative at that time. 2.The number of 104 or more CFU per milliliter required for detection of microorganisms in samples.And also, it was suggested that detection time were changed by kind of strains and mediums (culture bottles). Our results suggest that A. O. staining technique is supposed to be sensitive, effective screening method for the early detection of bacteremia but that in some cases, blind subculture and other supplementary methods are necessary when A. O. staining technique is used.
日本感染症学会の論文