病原性赤痢菌と非病原性赤痢菌の拮抗性について
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概要
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A radio-active Shigella bacillus was prepared by the cultivation of a virulent or an avirulent strain in H. I. Broth containing 5μ Ci of <SUP>32</SUP>P-orthophosphate (H<SUB>3</SUB><SUP>32</SUP>PO<SUB>4</SUB>). To clarify the invasion mechanism of these strains into host cells, following experiments were carried out.<BR>When the radio-active virulent strain was inoculated onto monolayer of HeLa cells, the radioactivity of the cells began to rise at 30 minutes after the inoculation, suggesting the beginning of invasion of the virulent strain, and thereafter the radioactivity increased with the lapse of time. On the other hand, the radioactivity of the cells inoculated with the radio-active avirulent strain showed a constant low level throughout the experiment, suggesting no invasion of the avirulent strain. At the simultaneous inoculation of the radio-active virulent and avirulent strains onto the HeLa cells, the radioactivity of the cells did not rise until 60 minutes after the inoculation and thereafter rose slowly up to 120 minutes. But on this case the radioactivity at 120 minutes showed a half of the level of the inoculation of the virulent strain only, suggesting the occurence of antagonism at a site of invasion between these two strains. This phenomenon did not occurred in the experiment with the virulent strain and the killed avirulent strain instead of the live one.<BR>When the radio-active strain, irrespective of virulent or avirulent on, was inoculated onto a monolayer of peritoneal adherent cells (macrophages) from guinea pigs, the radioactivity of the macrophages rose rapidly and reached to a peak within 30 minutes. Numbers of bacillus in the host cells were found to increase by the microscopic observation later than 30 minutes after inoculation, nevertheless the radioactivity did not increase thereafter. This might be considered not to the new invasion of radio-active bacteria, but to the multiplication of bacteria already taken in the cell. At the experiment of simultaneous inoculation with the virulent and the avirulent strains, the radioactivity of macrophages was the same level as that of the virulent strain only.
- 日本感染症学会の論文
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