緑膿菌臨床分離株と環境株の外毒素産生能について
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Previously, Pollack et al., Bjorn et al. and Sanai et al. reported that an incidence of the exotoxin producing <I>P. aeruginosa</I> was 87, 89 and 87 % among clinical isolates, respectively.<BR>In the present study, the exotoxin production of clinical and environmental isolates of <I>P. aeruginosa</I> was tested by an intradermal inoculation of their culture filtrates into any guinea pig and correlation between the exotoxin producibility of isolates and their isolating sources or serotypes was investigated.<BR>Exotoxin production was observed to be 79% among clinical isolates from patients with underlying diseases, while it was 22% positive from the environmental isolates of <I>P. aeruginosa</I>. Difference was significant at P<0.05. Actually, number of the exotoxin positive strains from different clinical source tested were 8 out of 12 isolates from urine, 12 out of 15 isolates from sputum, 9 out of 10 isolates from pus and 3 out of 5 isolates from others (bile, ear discharge and tonsil), suggesting not significant dependency upon the isolating sources.<BR>So far as the present results were concerned, as difinite relation of the exotoxin producibility to any serotype of the isolates was not observable, that is, there were 7 exotoxin positive strain out of 9 A type isolates (78%), 12 out of 13 B type isolates (92%), 1 out of 1 D type isolates, 2 out of 6 E type isolates (50%), 1 out of 2 F type isolates (50%), 6 out of 8 G type isolates (75%) and 3 out of 3 I type isolates (100%).<BR>However, a higher frequency of the exotoxin producing strains among both B and I serotypes of isolates was remained for further investigation.
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