Comparison of the Loop-Mediated Isothermal Amplification (LAMP) Method and Conventional Culture Method for the Detection of Campylobacter Species from Retail Chickens

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With the newly designed LAMP primers for C. jejuni and C. coli, we examined Campylobacter in 134 samples of retail chickens, and the usefulness of the LAMP method in comparison with a culture method. Twenty-four samples (17.9%) were positive, and 99 samples (73.9%) were negative in both test methods; the consistency of both tests was high (91.8%). Among the samples showing inconsistent results, 10 samples (7.5%) were positive in the LAMP method and negative in the culture method; in contrast, only 1 sample (0.7%) was negative in the LAMP method and positive in the culture method. No significant difference was noted when the detection status was compared by chi-square test. Analysis of Campylobacter according to the types of materials showed that 5 of 23 chicken wing samples (21.7%) were positive by the LAMP method, while none by the culture method. For others, no marked difference was noted for thigh, liver, ground meat, breast, and chicken fillet by both test methods. In this study, the LAMP method showed excellent results comparable to those by the culture method and thus was a very useful test method for food sanitation, considering its characteristics such as convenience and rapidity.
日本食品微生物学会の論文