Study of Simple and Rapid Detection of Salmonella spp. Using VIDAS
スポンサーリンク
概要
- 論文の詳細を見る
Both the incidence and number of patients with food-borne diseases caused by <I>Salmonella</I>still rank very high among total food-borne diseases, although both have been decreasing in recent years. The need for a simple and rapid alternative method of <I>Salmonella</I>testing is very high in the food industry because the conventional culture method includes two enrichment procedures and takes more than five days to isolate and identify a typical colony of <I>Salmonella</I> from samples. VIDAS is an enzyme-linked fluorescent assay (ELFA) system that consists of a fully automated device and its reagents. In this study, we compared two protocols of the VIDAS method with the conventional culture method for detecting <I>Salmonella</I> using 40 food samples. Performances of protocols " VIDAS ICS + plate "and " VIDAS SLM AFNOR validated method" both showed higher sensitivity than the conventional culture method. These protocols were also excellent in terms of reduced time-toresults in addition to showing higher sensitivity. We also compared SMID 2, a new chromogenic agar plate for selective isolation of <I>Salmonella</I>, with DHL agar, a traditional agar plate for <I>Salmonella</I> testing. On SMID 2, typical colonies of <I>Salmonella</I> were much easier to identify (mainly by its characteristic color), and the frequency of appearance of typical colonies by bacteria other than <I>Salmonella</I> was much lower, although the <I>Salmonella</I> recovery rate did not differ between the two media.
- 日本食品微生物学会の論文
日本食品微生物学会 | 論文
- 腸内細菌プロファイル : 見えてきたヒト腸内細菌叢の全貌
- 市販鶏肉から分離された Salmonella Enteritidis の疫学解析
- Vibrio vulnificus の清水港湾内における分布
- 腸管出血性大腸菌O157分離培地BD CHROMagar^ O157の評価
- ヒト, 家畜および食肉から分離されたカンピロバクターの薬剤感受性