Detection of Listeria monocytogenes in Natural Cheese by Enzyme-Linked Fluorescent Assay

概要

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The VIDAS Listeria monocytogenes 2 (LMO 2) is an enzyme-linked fluorescent assay (ELFA) system which is a fully automated ELISA technology to detect Listeria monocytogenes. This system is a powerful tool for the food industry because assay results following a 48 hr enrichment procedure can determine the presence or absence of L. monocytogenes within 70 min. The aim of this study is to compare the ELFA method with the conventional cultural method for detecting L. monocytogenes in natural cheese. We confirmed 33 samples (19 types) of natural cheese tested were not naturally contaminated by L. monocytogenes using both detecting methods. L. monocytogenes was then inoculated into the cheese samples at a concentration of 1cfu/g. Twenty of 33 samples showed 100% positive by the culture method. Twenty-seven samples showed 100% positive by the ELFA method. The overall positive rate of 33 samples of natural cheese were 83% and 89% for the culture method and ELFA method, respectively. Two samples of Blue cheese and one sample of Camembert cheese produced a poor positive rate by the ELFA method due to low numbers of L. monocytogenes cells after the enrichment procedure. These results show that the ELFA method could be used satisfactorily, except for particular cheeses, as a rapid method for the detection of L. monocytogenes in natural cheese inspection.
日本食品微生物学会の論文