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We evaluated the ability of the 5'-nuclease (TaqMan) PCR assay by Commercial Detection Kit to detect Shigatoxin-producing Escherichia coli O157 in pure cultures, inoculated raw ground meat, shrimp and vegetable enrichment samples. The results were compared with the culture method, standard PCR assay and ELISA. The TaqMan PCR was able to identify the organism when 102 cfu/ml or more organisms were present in pure culture and in novobiocin-modified EC enrichment of several food samples. In comparison, the recovery efficiency on CTSMAC plate was from 101 to 106 cfu/ml, standard PCR assay sensitivity was 104 cfu/ml or more organisms, and ELISA test showed false positive in contaminated samples of Citrobacter freundii. Our findings indicate that TaqMan PCR assay is a useful automatic assay for identifying the existence of E. coli O157 in food samples with a high detection sensitivity and results in two days.
日本食品微生物学会の論文