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At low levels, enterohemorrhagic <I>Escherichia coli</I> should be selectively isolated by suppressing competing microflora in meat samples. In conventional methods, MacConkey II Agar with C-T Sorbitol (cefixime-tellurite, CT-SMAC) which utilizes the ability of <I>E. coli</I> O157 to ferment sorbitol, and media containing <I>E.coli</I>-specific chromogenic substrates, are used for detecting <I>E. coli</I> O157.<BR>In this study, we compared two types of BD CHROMagar<SUP>TM</SUP> O157 for the isolation of enterohemorrhagic <I>E. coli</I> O157 (improved BD CHROMagar<SUP>TM</SUP> O157 and conventional BD CHROMagar<SUP>TM</SUP> O157) with CT-SMAC by using the Miles-Misra method and evaluating the recovers from ground beef and human fecal samples. The results obtained are described below: <BR>1. In the inoculation test with three media by the Miles-Misra method, improved BD CHROMagar<SUP>TM</SUP> O157 inhibited the growth of all organisms except <I>E. coli</I> O157 better than the two other media and allowed easy differentiation from <I>E. hermannii</I>, which could not be distinguished on CT-SMAC.<BR>2. In the <I>E. coli</I> O157 detection test for ground beef artificially inoculated with <I>E. coli</I>O157 at 1 cfu/g, the detection rate of improved BD CHROMagar <SUP>TM</SUP> O157 was 95%, CTSMAC 75% and conventional BD CHROMagar<SUP>TM</SUP> O157 40%, respectively.<BR>4. In the <I>E. coli</I> O157 detection test for <I>E. coli</I> O157 positive human fecal samples, the detection rate of improved BD CHROMagar<SUP>TM</SUP> O157 was 54.5%, CT-SMAC 50% and conventional BD CHROMagar<SUP>TM</SUP> O157 22.7%, respectively.
- 日本食品微生物学会の論文
日本食品微生物学会 | 論文
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