Detection of O157 and H7 Antigen Genes of Escherichia coli by PCR.
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概要
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A PCR method was developed for the simultaneous detection of <I>Escherichia coli</I> O157 and H7 antigens. Two PCR primer pairs for amplification of both <I>E. coli</I> O157 <I>rfbE</I> and H7 <I>fliC</I> genes, which are necessary for the expression of the O157 and H7 antigen respectively, were performed for the detection of E. <I>coli</I> O157: H7. All Shiga toxin-producing <I>E. coli</I> (STEC) O157: H7 and STEC O157: NM strains were positive for both E. coli O157 <I>rfbE</I> and H7 <I>fliC</I> genes.<BR>Non-STEC O157 strains were positive only for <I>E. coli</I> O157 <I>rfbE</I> genes and H7 strains except O157 were positive only for H7 <I>fliC</I> genes . Some of the nonmotile strains were positive for H7 <I>fliC</I> genes. No cross-reaction was observed with other <I>E. coli</I> serotypes (except O157 and H7) and other bacterial species, like Salmonella O30 and <I>Citrobacter freundii</I> which react with <I>E. coli</I> O157 antiserum. It is recommended that PCR amplification of both <I>E. coli</I> O157 <I>rfbE</I> and H7 <I>fliC</I> genes is one of the most specific methods for <I>E. coli</I> O157: H7 identification.
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