Comparison of the Commercial Locate Kit with Conventional Culture Method for the Detection of Salmonella in Raw Chicken.
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概要
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The commercial ELISA kit (Locate) was compared with a conventional culture method for the detection of <I>Salmonella</I> in foods. The sensitivity of the ELISA kit was detection of 10<SUP>6</SUP> cells/ml of <I>S. Enteritidis</I>, S. Tyhimurium and S. Infantis in pure culture. All of the 23 serovars of <I>Salmonella</I> tested gave correctly positive results, while the 7 strains of <I>C. frendii</I> gave correctly negative results.<BR>Raw meat and liquid egg which had been inoculated with low levels of different serovars of <I>Salmonella</I> gave positive results by the kit and culture method. In 50 samples of raw chicken, the correlation between the ELISA kit and culture method was very high, 98.0%. An ELISA false negative result was obtained in one sample. <I>Salmonella</I> recovered from the enrichment broth was not reactive with the ELISA, because the organism was covered with mucoid substance. The ELISA kit was found to be a rapid screening method for the detection of <I>Salmonella</I> in food.
- 日本食品微生物学会の論文
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