Evaluation of Q-TROL for the Rapid Detection of Salmonella from Food Samples

概要

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Q-TROL is an enzyme immunoassay (EIA) kit for detecting Salmonella antigens in foods by use of monoclonal antibodies against Salmonella coated on wells of plastic trays. Q-TROL was compared with the conventional method for detecting Salmonella from the same meat samples in three laboratories by the same methods. A preliminary study was performed to improve the original method described in the manual for saving time and for simplifying the procedure. As the results, it was found that the incubation in M broth at 35°C for 6 h followed by enrichemnt culture in SBG at 43°C for 18 h could be omitted, and that SBG was most effective among various selective enrichment media. One hundred chicken meat samples were analysed by both the methods, conventional and EIA, in three laboratories (A, B and C), and other 94 samples of a variety of food in laboratory C. There was a good correlation in the samples positive by both the methods without any false negative, but there were a few false positives. The EIA screening method has been regarded as a rapid and reliable method for detecting Salmonella.
日本食品微生物学会の論文