Bacteriostatic Effect of Biocon®, a Food Preservative of an Aerosol Mixture of Ammonium Bicarbonate, Sodium Chloride and Carbon Dioxide

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Biocon®, an aerosol mixture of ammonium bicarbonate, sodium chloride and carbon dioxide, was examined for the effectiveness as a food preservative. Flushing the aerosol on culture media suppressed the colony formation by Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis and Candida albicans. The inhibition of the growth occurred when those organisms were inoculated either before or after the flushing on agar plates. The effect was bacteriostatic and it remained active for 10 days if the media plates were sealed properly and kept at 0-4° after the flushing. The active component was proved to be NH4HCO3, which kept the reaction of the surface of agar plates between pH 8.6-9.0, most probably by maintaining a certain equilibrium among the gas-liquid state of NH4HCO3, NH3 and CO2. Biocon gave similar effects on sliced meat for 5 days without affecting taste, smell or color, if the initial contaminating bacterial number was less than 105 CFU/g. Biocon when handled properly seemed to be a harmless preservative to keep freshness of raw meats.
日本食品微生物学会の論文