Studies on Genetic Differentiation of <I>Salmonella</I> Haifa Isolated from Salmonellosis and Chicken Meat by Plasmid Profile
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概要
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A total of 180 meat samples, consisting of 60 beef, 60 pork and 60 chicken obtained at three butchers in Shimane prefecture, were examined for the presence of <I>Salmonella</I> during the period from June, 1991 to March, 1992.<BR>The results were as follows.<BR>1) <I>Salmonella</I> were found in 1 (1.7%) of 60 beef, 4 (6.7%) of 60 pork and 24 (40.0%) of 60 chicken. The rates of detectiòn was highest for chicken.<BR>2) The population of <I>Salmonella</I> varied under 10 cfu/100 g in beef and pork, under 10<SUP>2</SUP> cfu/100 g in chicken. Chicken tended to be somewhat more heavily contaminated with <I>Salmonella</I> than beef and pork.<BR>3) Thirteen-six strains isolated from these meat were classified into 11 serotypes. Main detected serotype was S. Haifa (9 strains: 25.0%), S. Typhimurium (7 strains: 19.4%) and S. Virchow (5 strains: 13.9%).<BR>4) The plasmid profile of S. Haifa from 14 strains (from 9 strains of chicken, 1 strain of food poisoning and 4 strains of salmonellosis) showed 6 groups. First group, having 4.2 Md and 2.6 Md of plasmid DNA, was isolated from 3 strains of chicken, 1 strain of food poisoning and 1 strain of salmonellosis. Second group, having 4.8 Md and 2.9 Md of plasmid DNA, was done from 1 strain of chicken 2 strains of salmonellosis. Third group having 9.2 Md, 4.2 Md, 3.7 Md, 2.9 Md and 2.6 Md of plasmid DNA, was done from 2 strains of chicken and 1 strain of salmonellosis.<BR>This is suggested that food poisoning happens from chicken meat polluted with S. Haifa in Shimane prefecture and the plasmid profile is valuable for the epidemiological examination as a maker.
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日本食品微生物学会 | 論文
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